+4 °C’de Krebs-Henseleit Solüsyonunda Soğuk Depolamanın Sıçan Torasik Aortunun Vazoreaktivitesi Üzerindeki Etkileri
نویسندگان
چکیده
Aim: This study aimed to investigate whether endothelium-dependent and -independent relaxation responses can be preserved intact in the rat thoracic aorta after storage for 3, 6, 18 hours Krebs-Henseleit solution at +4 °C.
 Methods: Isolated organ bath model 10-12 weeks old male Wistar rats were used perform experiments. To effect of factors, cyclooxygenase inhibitor INDO was added inhibit endogenous prostanoid synthesis. Submaximal contraction response obtained with a single dose PE then ACh administered cumulatively (10-9-10-4 M) induce responses. Besides, smooth muscle-dependent by applying SNP (10-9-10-5 following precontraction induced PE. The statistical significance level considered as p0.05). did not cause significant change (p>0.05). Similarly, modulate endothelium-independent aortic segments or (p>0.05).
 Conclusion: In present study, first physiological findings have been that contraction-relaxation aortas periods +4°C.
منابع مشابه
Myocardial protection against global ischemia with Krebs-Henseleit buffer-based cardioplegic solution
BACKGROUND The Krebs-Henseleit buffer is the best perfusion solution for isolated mammalian hearts. We hypothesized that a Krebs-Henseleit buffer-based cardioplegic solution might provide better myocardial protection than well-known crystalloid cardioplegic solutions because of its optimal electrolyte and glucose levels, presence of buffer systems, and mild hyperosmolarity. METHODS Isolated L...
متن کامل1,2,3,6,7,8-Hexahydrocinnolino[5,4,3-cde]cinnoline
The title compound, C(12)H(12)N(4), was synthesized by the reaction of hydrazine hydrate and 9-methyl-3,4,6,7-tetra-hydro-2H-xanthene-1,8(5H,9H)-dione in ethanol. In the crystal, the mol-ecule lies across an inversion centre. The pyridazine rings are coplanar and the C(6) rings adopt envelope conformations.
متن کاملElectrical field stimulation causes oxidation of exogenous histamine in Krebs-Henseleit buffer: a potential source of error in studies of isolated airways.
Electric field stimulation (EFS) relaxes human histamine-precontracted airways in vitro. This relaxation is only partly neurally mediated. Nonneural relaxation has been also shown in blood vessels and is due to the generation of oxygen radicals by EFS. In isolated airways the origin of the nonneural component of the relaxation is not clear. Because exogenous catecholamines are oxidized during E...
متن کاملCardiomyocyte Apoptosis Induced in Langendorff Preparation of Isolated Guinea-Pig Heart Perfused with Krebs-Henseleit Solution Deprived of Glucose, with and without Oxygen Supply
Whether or not deprivation of glucose and oxygen can induce cardiomyocyte apoptosis was examined. Four groups of Langendorff preparations of guinea-pig hearts were perfused for 6 h with Krebs-Henseleit (K-H) solution deprived of glucose (group A), deprived of oxygen (group B), and deprived of both glucose and oxygen (group C), then examined for the mode of myocardial cell death. The control was...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Bal?kesir sa?l?k bilimleri dergisi
سال: 2022
ISSN: ['2146-9601']
DOI: https://doi.org/10.53424/balikesirsbd.1141819